p akt ser473 (Cell Signaling Technology Inc)

Cell Signaling Technology Inc is a verified supplier

Cell Signaling Technology Inc manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Cell Signaling Technology Inc p akt ser473
P Akt Ser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 17252 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+akt/Phospho-Akt+(Ser473)+XP+Rabbit+mAb/pm42892247-71-17-20
Average 99 stars, based on 17252 article reviews

p akt ser473 - by Bioz Stars, 2026-11

99/100 stars

Images

Western Blot:

Article Title: Cucurbitacin B attenuates LPS-induced skeletal muscle atrophy by inhibiting JAK2/STAT3 and suppressing proteasome activity.
Article Snippet: .. Main primary antibodies used for Western blotting: MyoD (Novus Cat. no. NBP1–54153), MyHC (Abcam Cat. no. ab37484), p-IGF-1R (CST, Cat. no. 3918 T), IGF-1R (CST, Cat. no. 9750 T), IRS-1 (CST, Cat. no. 3407 T), p-Akt (CST, Cat. no. 4060),Akt (SC, Cat. no. Sc-5298), p-mTOR (CST, Cat. no. 2971), mTOR (SC, Cat. no. Sc-8319), p-GSK3β (CST, Cat. no. 5558), GSK3β (SC, Cat. no. Sc-53,931), p-p70S6K (CST, Cat. no. 9204), p70S6K (SC, Cat. no. Sc-8418), p-4E-BP1 (CST, Cat. no. 9451), 4E-BP1 (SC, Cat. no. Sc-6936), FoxO3a (CST, Cat. no. 12829S), Lamin B (Absin Cat. no. Abs131244), Atrogin-1 (Novus Cat. no. NBP1–79517), MuRF1 (SC, Cat. no. Sc-398,608), LC3B (CST, Cat. no. 43566 T), Beclin1 (CST, Cat. no. 3495), P62 (Abmart, Cat. no. T55546F), β-actin(Absin Cat. no. abs32001), p-STAT3 (Tyr 705) (CST, Cat. no. 9145), p-STAT3 (Ser 727) (CST, Cat. no. 9134), STAT3 (SC, Cat. no. Sc-8019), p-JAK1 (CST, Cat. no. 3331), JAK1 (CST, Cat. no. 3332), p-JAK2 (CST, Cat. no. 3776), JAK2 (CST, Cat. no. 34315), p-Src (CST, Cat. no. 2105), Src (CST, Cat. no. 2109), IL-1β (ABclonal,Cat. .. no. A16288),TNF-α (Abmart, Cat. no. PA2174F).

Article Title: GDNF-Enriched Small Extracellular Vesicle-Loaded ROS-Scavenging Hydrogel Promotes Neural Regeneration After Spinal Cord Injury.
Article Snippet: .. Total proteins were extracted from spinal cord tissues, and equal amounts of protein were subjected to Western blot analysis using antibodies against PI3K (1:1000; 4257; CST), p-PI3K(1:1000; 4228; CST), AKT(1:1000; 9272; CST), and p-AKT(1:1000; 9271; CST). .. Protein bands were quantified using ImageJ, and activation of the PI3K/Akt pathway was evaluated by calculating the p-PI3K/PI3K and p-AKT/AKT ratios.

Incubation:

Article Title: KLF4 Protects Against Sepsis-Induced Coagulopathy via Inhibition of the AKT/mTOR/STAT3 Signaling Pathway
Article Snippet: .. The PVDF membranes were blocked with 5% skimmed milk for 2 h at room temperature, followed by incubation with thrombin (ab314480, 1:1000, Abcam), PAI-1 (ab222754, 1:1000, Abcam), TF (DF6400, 1:2000, affinity), GAPDH (AF7021, 1:5000, affinity), Tubulin (AF7011,1:1000, affinity), p-AKT (4060S, 1:2000, CST), AKT (4691S, 1:1000, CST), p-mTOR (5536S, 1:2000, CST), mTOR (2983S, 1:1000, CST), KLF4 (4038T, 1:1000, CST), p-STAT3 (9145T, 1:2000, CST) and STAT3 (9139T, 1:1000, CST) were incubated at 4°C overnight. .. The next day, the membranes were incubated with HRPlabeled affinity-pure goat anti-rabbit IgG (H+L) (SA00001-2, 1:15000, Proteintech) or HRP-labeled affinity-pure goat anti-mouse IgG (H+L) (SA00001-1, 1:15000, Proteintech) for 1 h at room temperature.

Article Title: A multifunctional injectable MPDA@MTIC–Co hydrogel platform for synergistic chemotherapy–photothermal therapy of postoperative glioblastoma
Article Snippet: .. Membranes were incubated with primary antibodies against PI3K, AKT, and p-AKT (Cell Signaling Technology) overnight, followed by HRP-conjugated secondary antibodies. .. Signals were detected using ECL reagent (Thermo Fisher), with GAPDH as loading control.

Cell-Signaling:

Article Title: Ephedrae Herba and Armeniacae Semen Amarum combination alleviates LPS-induced acute lung injury by targeting the TLR4/NF-κB and NLRP3 pathways.
Article Snippet: ALI is a severe clinical syndrome with limited treatment options beyond corticosteroids, which cause significant adverse effects.. This study demonstrates that the herbal combination EAC significantly attenuates LPS-induced ALI through dual pathway inhibition.. EAC suppresses TLR4/NF-κB and NLRP3 inflammasome signaling, thereby inhibiting MyD88-mediated NF-κB activation, NLRP3 inflammasome assembly, caspase-1 activation, and GSDMD|mediated pyroptosis.

Magnetic Resonance Imaging:

Analysis:

other:

Article Title: Integrated cryopreservation-thawing-transplantation platform for neural stem cell-based spinal cord injury repair
Article Snippet: The cryoprotective solution was prepared in advance with the specific formula: 10 % dimethyl sulfoxide (DMSO, Sigma-Aldrich, USA), 20 % fetal bovine serum (FBS, Gibco, USA), and 70 % complete medium (DMEM/F12 + Glucose + penicillin/streptomycin).

Article Title: Endothelial Nitric Oxide Synthase Restores Diabetic Dentin Regeneration via AKT/Endothelial Nitric Oxide Synthase Axis
Article Snippet: Protein concentration was determined using a bicinchoninic acid assay kit (P0012, Beyotime).

Review